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Fig. S1
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Fig. S1
RNA detection for genetic markers of osteoblasts in juvenile animals assessed using RNAscope in situ hybridization on paraffin section.
(A-I) Sequential sections (4 μm) of the interfrontal suture collected from juvenile zebrafish at age of 6 wpf. The expression of individual genes is visualized in red, counterstained with haematoxylin for nuclei in purple; black arrows indicate assumed positive expression. The expression of following genes is shown: (A) col2a1a, (B) runx2a, (C) spp1, (D) fgfr1a, (E) fgfr1b, (F) fgfr2, (G) twist2, (H) twist3, (I) foxq1a. The scale bar represents 20 μm.
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