ZFIN Image: Katz et al., 2016, Fig. 3

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Figure Caption

Fig. 3

miR-9 Potentiates Notch Activity to Maintain Quiescence

(A and B) Boxplots of qRT-PCR analysis showing the relative change in expression of the miR-9 transcripts and the Notch target her4 (A) and of the mature miR-9-5p/3p compared to a U6 small nucleolar RNA (snoRNA) control (B) after a 48 hr treatment with LY411575 or DMSO (control). The top and the bottom of each box indicate the 75^th and 25^th percentiles, respectively, and the dotted line indicates the median.

(C) Expression of miR-9 (magenta) in a GS⁺ (green)/PCNA⁺ (light blue) aNSC (yellow arrow) after a 48 hr LY treatment. Scale bar, 5 μm.

(D) Proportion of qNSCs (gfap⁺, MCM5⁻; dark gray) and aNSCs (gfap⁺, MCM5⁺; white) in the miR-9⁺ or miR-9⁻ NSC population after a 48 hr treatment with LY or DMSO.

(E) ISH for her4 2 dpi with the control (top) or miR-9 (bottom) vivoMO. Right panels are close-ups of the region within the red box. Scale bars, 100 μm (left); 50 μm (right).

(F) Epistasis experiment combining miR-9 kd with overexpression of Notch intracellular domain (NICD). The graph shows the percentage of qNSCs (dark gray) and aNSCs (white) in brains injected with the control or miR-9 vivoMO and in cells overexpressing NICD (NICD⁺) or not (NICD⁻). ^∗p < 0.05, ^∗∗p < 0.01; one-way ANOVA with Bonferroni post hoc correction. Data are represented as mean ± 95% CI; n = 3 brains per condition.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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