ZFIN Image: Taylor et al., 2016, Fig. 4

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Figure Caption

Fig. 4

Colocalization of ret and sox10 with phox2bb reveals a total of three enteric progenitor subpopulations. A and C show confocal images of cross-sections through the trunk. At 54 (A) and 60 (C) hpf, phox2bb (green), ret mRNA (red) and sox10 mRNA (blue) colocalize in subpopulations. Insets show close-up of outlined cell(s), overlay, phox2bb, ret mRNA, sox10 mRNA (from left to right). Note that phox2bb refers to phox2bb:EGFP expression as described in the text. At 54 (B) and 60 (D) hpf, quantification in percent of phox2bb, ret and sox10 colocalization in the three regions analyzed. The dotted line between the measured values does not show the continuous percentage of coexpression between the anterior, mid and posterior values, but is drawn to facilitate visualization of coexpression trends. ie, intestinal epithelium. Scale bar = 10 μm.

Acknowledgments

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