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Fig. S2
Double immunofluorescence stainings with anti-Kir4.1 pAb (red) and anti-synt mAb (green) in U251 cells expressing WT (upper panels) or R18Q (lower panels) Kir4.1 reveal a partial colocalization of Kir4.1 and syntrophin in the plasma membrane and in the cytoplasm of both astrocytoma cell lines. Compared to Kir4.1 WT expressing cells, a larger number of R18Q+ cells shows colocalization of syntrophin and Kir4.1 in both cytoplasm and plasma membrane (arrowheads). Scale bars: 10 µm.
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