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Fig. 5
stat1 gene expression assayed by RT-PCR. A. Calibration series. A gradient of RNA dilutions and 21 amplification cycles with stat1b primers verified a semi-quantitative analysis of target mRNAs. B. Detection of stat1a and stat1b expression using RT-PCR at various stages of development using beta actin expression as loading control. RNA samples were extracted from zebrafish embryos at the indicated ages. Abbreviations: -, negative control with no RNA added; u, unfertilized eggs; 0.5, 2, etc., ages at hours post fertilization (hpf).
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Reprinted from Mechanisms of Development, 128(7-10), Song, H., Yan, Y.L., Titus, T., He, X., and Postlethwait, J.H., The role of stat1b in zebrafish hematopoiesis, 442-56, Copyright (2011) with permission from Elsevier. Full text @ Mech. Dev.