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Fig. S9

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ZDB-IMAGE-160923-15
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Figures for Qiu et al., 2016
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Fig. S9

GFP expression pattern in Tg(pax1a:gfp) transgenic line. This line was identified from the founder fish that was injected with the Tol2(pax1a:gfp) construct at the one-cell stage. The expression of GFP was driven by a 2.4-kb promoter of the pax1a gene. (A, E and I) in situ hybridization indicated the expression of endogenous pax1a in the sclerotome of wild-type embryos at 24 hpf (A), 30 hpf (E) and 48 hpf (I). (B-D) GFP expression in transgenic embryos at 24 hpf. A trunk region (C) and an optical cross section (D) of the same embryo (B) were shown. n, notochord. (F-H) GFP expression in transgenic embryos at 30 hpf. A trunk region (G) and an optical cross section (H) of the same embryo (F) were shown. (J-L) GFP expression in transgenic embryos at 48 hpf. A trunk region (K) and an optical cross section (L) of the same embryo (J) were shown. (M) A GFP+ circulating blood cells (indicated by arrows) was observed by confocal microscopy in the heart at 36 hpf. (N) Flow cytometry result showing the proportion of GFP+ blood cells in embryos at 28 hpf. Blood cells were taken from the heart of 5-10 embryos at 28 hpf. The average in parenthesis was the mean of three experiments. (O) Flow cytometry results showing the proportion of GFP+ cells in adult blood (left) and kidney marrow (right). The showed were representative results from single fish. The average from three fish was shown in parenthesis.

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Mol. Cell Biol.