ZFIN Image: Tuttle et al., 2014, Fig. 2

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Figure Caption

Fig. 2

Rbc3a loss of function disrupts NC migration.

(A-C) Live 24 hpf controls (A), rbc3a-MO1-injected (B), and rbc3a^Q850X mutant (C) embryos. Morphant/mutant morphological defects include the mhb and cell aggregates in the dorsal midline (black arrows in B and C). (D-E′) NC defects in live sox10:gfp transgenics injected with rbc3a-MO1. (D, E) Merged bright-field and fluorescent images of the cranial region at 24 hpf, lateral views, show GFP+ NC cells accumulated dorsally (white arrowheads). (D′, E′) Dorsal views showing midline position of aggregates over the mhb and further posteriorly (white arrowheads). (F-Q) Whole mount in situ hybridization for markers of different NC lineages in controls (row 1), rbc3a-MO1-injected (row 2), and rbc3a^Q850X mutants (row 3) at 28 hpf, dorsal views, anterior to the left. dlx2 (F-H) expression in skeletogenic NC and foxd3 (I-K) expression in gliogenic NC appear unaffected, while mitfa in presumptive melanocytes (L-N) and gch in xanthophores (O-Q) are expressed in dorsal midline aggregates in rbc3a morphants/mutants (white arrows). Abbreviations: 1-4, pharyngeal arches; ot, otic vesicle; mhb, midbrain-hindbrain boundary. Scale bars, 100 µm.

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Acknowledgments

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