ZFIN Image: Chang et al., 2016, Fig. 2

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Figure Caption

Fig. 2

Leptospira Shermani induced acute tubular injury in zebrafish larvae.

Transverse histological sections (H&E stain) from control (A,D,G), L. Patoc-treated (B,E,H), and L. Shermani-treated larvae (C,F,I). Note the marked swelling of the proximal pronephric ducts (circles) in L. Shermani-treated larvae compared with L. Patoc-treated larvae and controls. The differences were not evident in the distal pronephric ducts. Living larvae were incubated in E3 media containing L. Shermani or L. Patoc (1 × 10⁶ CFU/ml) from 24 hpf to 48 hpf. Control larvae were incubated with E3 buffer only. Representative micrographs from the level of the glomerulus (A-C), proximal pronephric duct (D-F), and distal pronephric duct (G-I) are shown. Circles indicate the location of the pronephric duct. Pt, pronephric tubule; Glm, glomerulus; Scale bar, 10 µm. (J,K) Quantification of the area of pronephric ducts. *P < 0.05 compared to control. n = 6 from three larvae in each group. (L) Diagram of transverse sections illustrating the structure of zebrafish pronephros at 48 hpf.

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Acknowledgments

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