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Fig. 5
BMP pathway upregulates runx2 expression during opercle formation. (a) Embryos of Tg(fli1: gal4-vp16) were treated with or without 20 µM DMH1 from 54 to 72 hpf, fixed, and WISH was performed to detect the mRNA of runx2a. (b) Tg (runx2enhancer: gal4-vp16): (UAS: kaede) were treated with or without DMH1 from 54 hpf, irradiated with UV at 60 hpf, and Kaede-green intensity in the opercle area was measured at 72 hpf. Representative images are shown with pseudo color. Average and SD of Kaede-green intensity were calculated from more than 22 embryos in each sample, and are shown in right panel.
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