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Fig. 2
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Fig. 2
Overexpression of Hoxb8a leads to down-regulation of cxcr7b. (A) Fluorescent images of cldnb:lyngfp in cldnb4.2:gal4 x UAS:Hoxb8aMT embryos and control siblings at 45 hpf. The blue arrowhead shows the yolk defect seen in all cldnb4.2:gal4 x UAS:Hoxb8aMT embryos. Red arrowheads indicate the position of the primordium. (B) Quantification of the migration at 45 hpf (control, n = 20; cldnb4.2:gal4 x UAS:Hoxb8aMT, n = 26). Expression of (C) cxcr4b, (D) cxcr7b, and (E) lef1 in cldnb4.2:gal4 x UAS:Hoxb8aMT and control siblings at 28 hpf. ISH was followed by Myc immunostaining (brown). (Scale bar: 25 µm.)
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