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Fig. 3

Selected candidate genes are expressed in Hcrt neurons.

(AI′′) Double fluorescent staining of the candidate genes (red) and EGFP (green) was performed in 2 dpf hcrt:EGFP larvae using whole-mount ISH and immunofluorescence, respectively. White arrows indicate representative co-expressing cells. All confocal images show single plane view of 0.5 µM width. (J–M′′) Double fluorescent ISH and immunofluorescence experiments in brain sections of hcrt:EGFP adult zebrafish. Co-localization of candidate genes (red) and EGFP (green) in Hcrt neurons is shown. All images show single plane view of 0.5 µM width.

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Acknowledgments
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