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Fig. 2
Expression of disc1 at 50hpf is up-regulated around the midline of the hindbrain in response to increased Shh signalling. Expression was analysed using whole mount in situ hybridization. Dorsal, anterior to left (A,C) and transverse sectional (B,D) views show increased disc1 expression around the midline of the hindbrain in ptch1;ptch2 mutants (C,D) compared with siblings (A,B). Arrows indicate hindbrain midline disc1 expression in transverse sectional views.
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