ZFIN Image: Moreno-Ayala et al., 2015, Fig. 6

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Figure Caption

Fig. 6

Analysis of markers at mid-gastrulation. 75% Epiboly stage embryos. (A and B) Decreased Zimp7 activity causes a reduced signal in the ventrolateral mesoderm marker eve1 (arrows), and up-regulation of the neuroectodermal marker otx2 (C and D). (E and F) Expression of the endoderm and dorsal forerunner cells (DFCs) marker, sox32. Although comparable numbers of endodermal cells are detected in the morphants, the endodermal precursor cells are delayed relative to the DFCs (bar), which remain normal. (G and H) Sytox stained embryos. Similar migration defects are observed, and a bigger gap between the deep layer cells and the enveloping layer cells are observed in the zimp7 morphants relative to the control. eve1, vegetal views, dorsal to the right. otx2 animal views, dorsal to the right. (E–H) lateral views, animal to the top. (A, C, E, and G) control embryos and (B, D, F, and H) zimp7 morphants are shown. I, total number of embryos examined (indicated at the bottom) and the mean expression area differences between Ctrl MO and zimp7-sp MO injected embryos shown in A–D. The number of embryos with given appearance for sox32 (F) is 77% (n=22) and for Sytox 100% (n=5). ^***p<0.001.

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Acknowledgments

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Reprinted from Developmental Biology, 403(1), Moreno-Ayala, R., Schnabel, D., Salas-Vidal, E., Lomelí, H., PIAS-like protein Zimp7 is required for the restriction of the Zebrafish organizer and mesoderm development, 89-100, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.