ZFIN Image: Lush et al., 2014, Fig. 9

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Figure Caption

Fig. 9

Pharmacological activation of Wnt/β-catenin signaling is sufficient to induce interneuromast cell proliferation.

To verify BIO induces Wnt/β-catenin signaling we treated 48 hpf zebrafish with DMSO or BIO for 6 hr and then fixed and stained for lef1 expression. Compared to DMSO treated fish, BIO induces expression of the Wnt/β-catenin target lef1 in neuromasts and interneuromast cells (A–B). The large cluster of cells that are labeled in both (A) and (B) is primII (arrowhead). To measure proliferation Tg(SqET20:gfp) fish were treated with BrdU plus DMSO or BIO at 48 hpf and fixed at 72 hpf. (C–E) DMSO treated interneuromast cells (arrow) show single chain morphology with rare BrdU incorporation. BrdU incorporation is strong in primary neuromasts. (F–H) BIO treated fish show increased interneuromast cells with BrdU incorporation (arrows). Quantification of BrdU index (I, Student’s t-test, p=0.0003) and Tg(SqET20:gfp) positive cell number (J, Student’s t-test, p=2.75 E⁵) shows a significant increase in both after BIO treatment.

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Acknowledgments

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