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Fig. 4
Her2 regulates neural progenitor proliferation. (A) The expression of neural progenitor markers sox2 and sox3 was downregulated in Her2-knockdown embryos and was rescued by co-injection with her2 cRNA. (B) her2 induced the expression of sox2 and sox3. (C and D) The results of in situ hybridization in A and B were quantitatively confirmed by qPCR analysis, respectively. (E, F) Proliferation of neural progenitors was detected by in situ hybridization using a sox2 riboprobe (purple) and counterstaining with phosphohistone H3 antibody (brown). The bottom panels are representative of the enlarged regions of the upper panels, as indicated. (G, H) The proportions of the phosphohistone H3- and sox2-positive cells among the total sox2-positive cells were quantified (left panels in G and H), showing that Her2 knockdown resulted in the decreased proliferation of sox2-positve neurons (left panel in G) and that her2 cRNA induced the proliferation of sox2-positive neurons (left panel in H). The right panels in G and H show the quantification of the proportions of phosphohistone H3-positive and sox2-negative cells in sox2-negative cells that were counted in the adjacent surface ectoderm, which showed no significant deviation in Her2-distorted embryos. (I, J) The sox2-positive region was quantified by ImageJ. All panels show embryos at 75% epiboly. pH3, phosphohistone H3; *, p<0.05; **, p<0.01; n.s., not significant.
Reprinted from Developmental Biology, 397(1), Cheng, Y.C., Chiang, M.C., Shih, H.Y., Ma, T.L., Yeh, T.H., Huang, Y.C., Lin, C.Y., Lin, S.J., The transcription factor hairy/E(spl)-related 2 induces proliferation of neural progenitors and regulates neurogenesis and gliogenesis, 116-28, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.