ZFIN Image: Kanagaraj et al., 2014, Fig. 2

Image

Figure Caption

Fig. 2

Suf/Spastizin expression analysis.

(A) Real-time PCR of suf/spastizin (red) and gdf9 (grey) mRNA during oogenesis relative to expression in the one-cell embryo normalized to gapdh and odc1 mRNA. Gdf9 is exclusively expressed in germ cells, which acts as a positive control for the purification of oocytes particularly in stage Ib oocytes, which are frequently contaminated with somatic follicle cells [111]. (B) suf/spastizin mRNA levels during embryogenesis, (C) in sexually mature adults, (D) and ovaries of different genotypes. (E) Conventional RT-PCR showing the expression of exon 34–36 (610 bp) of suf/spastizin mRNA at selected stages (h: hours post fertilization; d: days post fertilization). A lack of exon 35 is predicted to amplify a 413 bp product, which is not observed at any stage of oogenesis or embryogenesis. A longer run of this gel highlighting the 25 bp shorter transcript caused by the change of the splice donor in the mutant controls is shown in Figure S2. (F) Confocal section of wild-type (wt) and mutant (suf) stage III oocytes labeled with Suf/Spastizin antibody (green) and counterstained with lysotracker (red). Scale bar: 50 µm. Inset in wt panel shows the architecture of the oocyte cortex with the cytoplasm of the oocyte (yellow) forming microvilli crossing the acellular chorion membrane (CM; grey). The oocyte is surrounded by a layer of follicle cells (FC; green) [modified after 94].

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.