Fig. 1

Fig. 1 Expression of nr2f2 in developing veins in the zebrafish. (A) Schematic diagram of a 1 dpf zebrafish (modified from Kimmel et al., 1995)) with a red box showing the approximate position of images in B–G. (B–D) Whole mount in situ hybridization of the trunks of 24 hpf (B) and 48 hpf (C and D) zebrafish embryos probed for nr2f2. (E–G) Two-color fluorescent in situ hybridization of the trunk of a 24 hpf animal probed for kdrl (in red; panels E and G) and nr2f2 (green; panels F and G). Images are all lateral views of the trunks of whole mount stained animals (anterior to the left) except for panel D, which shows a transverse thick section through the trunk (the red line in panel C indicates the plane of section shown in panel D). Arrows in panels B, D, and G point to posterior cardinal vein expression of nr2f2, while arrowheads in panels D and G note the position of the dorsal aorta. Rostral is to the left and dorsal is up in all image panels. Scale bars, (B, C, E, F, and G) 100µm, (D) 50µm.