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Fig. 1 In situ hybridization of 30-somite stage wild-type embryos using markers of arterial-venous specification. (A) Line drawing of a 30-somite stage embryo. The boxed region corresponds to the area shown in B,D,F and H (modified from Kimmel et al., 1995). (B,D,F,H) Lateral views of (B) ephrinB2, (D) notch3, (F) flt4 and (H) fli1 expression in the trunk vessels. (C,E,G,I) Thick sections (~35-50 mm) through the trunk of embryos showing expression of (C) ephrinB2, (E) notch3, (G) flt4, and (I) fli1. (B-I) The dorsal aorta (DA) is indicated by a red arrowhead and the posterior cardinal vein (PCV) by a blue arrowhead. (E) Pronephric duct expression of notch3 is indicated by black arrowheads. NT, neural tube; NC, notochord. Scale bars, (A) 100 μm, (B,D,F,H) 40 μm, (C,E,G,I) 30 μm.