The clo mutant heart expresses normal levels of myofibrillar proteins. A number of myofibrillar proteins were examined by immunohistochemistry, including different isoforms of myosin heavy chain, troponins I and T, and tropomyosin. Comparable levels of tropomyosin as observed by immunofluorescence are seen in wild-type (A), and clo mutant (B) embryos at 36 hours. Arrows point to the heart and the other staining is skeletal muscle in the trunk.
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