Fig. 8 Expression of otpa in relation to gabaergic, glutamatergic and glycinergic markers in the hindbrain of wildtype larvae.
Potential coexpression of otpa with gabaergic (gad2, A), glutamatergic (vglut2, B) and glycinergic (glyt2, C) markers was analyzed by double whole mount FISH at 3 dpf. A1, B1, and C1 are single plane dorsal views of the hindbrain, anterior is to the left. A2, B2, and C2 are cross-sections at the level of the hindbrain indicated by the white line in A1, B1, and C1, respectively. The orthogonal view cross sections were obtained from dorsal confocal stacks using the TransformJ Turn plugin of the ImageJ software. Scale bar is 50 μm.