ZFIN Image: Novikov et al., 2013, Fig. 7

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Figure Caption

Fig. 7

Loss of Tmem88a reduces the CP fields. Zebrafish embryos were analyzed at the 8-somite stage. (A,B) WISH for nkx2.5 (box) and ntl in representative control and tmem88a morphants. (C) Relative nkx2.5⁺ areas in control and tmem88a morphants. (D) nkx2.5 relative expression levels as assessed by qPCR. (E,F) WISH for gata4 and ntl in representative control and tmem88a morphants. Arrow indicates the posterior gata4⁺ domain boundary. (G) Relative distance between the notochord tip and the posterior gata4⁺ domain boundary. (H) gata4 relative expression levels by qPCR. (A,B,E,F) Dorsal views of flatmount embryos, with anterior to the top; n>20 embryos analyzed per condition. (C,G) Measurements are normalized to control; n=10 embryos measured per condition. ^**P<0.01; ns, not significant; error bars indicate s.d.

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Acknowledgments

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