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Fig. 1
Cardiac morphology.
Heart positions are indicated by the arrowheads. (A–D) Cardiac morphology visualized by mRFP expression derived from Tg(cmlc2:mRFP). All images show ventral views at 28hpf. (E–L) Whole-mount in situ hybridization with amhc and vmhc RNA probes. All images show ventral views at 30hpf except for panel L (dorsal view). Genotyping was performed by genomic sequencing after taking pictures, wt (A,E,I), fn mutant (B,F,J), spns2 mutant (C,G,K) and fn;spns2 double mutant (D,H,L). Scale bars: 200μm. (M) Average distances between two hearts from multiple experiments; error bars represent standard deviations.
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