ZFIN Image: Drummond et al., 2013, Fig. 3

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Fig. 3

Zic2a and Zic2b depletion causes down-regulation of the retinoid-metabolism genes, cyp26a1 and aldh1a2, during early embryogenesis. mRNA in situ hybridization analysis of cyp26a1(A, B) and aldh1a2(C, D, F-I) expression reveals a reduction in both genes in Zic2a;Zic2b;p53 morpholino injected embryos (B, D, G, I) compared to control p53-morpholino injected embryos (A, C, F, H). Relative aldh1a2 mRNA expression levels for 7 hpf embryos were determined using quantitative real-time PCR (E). Levels reflect average of 7 technical replicates, with aldh1a2 mRNA at 0.71 significantly reduced (SD = 0.11; *p < 0.0001) compared to normalized control. Images are of 7 hpf embryos in dorsal view with anterior oriented to top (A-D), or lateral views with anterior to the left of 18 hpf (F, G) or 24 hpf embryos (H, I). Error bars depict standard deviation.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.