Fig. 2

Fig. 2

The maintenance of krox20 and mbp expression does not require Gpr126 signaling. (A) RT-PCR from dissected PLL nerves from 3-month-old gpr126^st49/+ and gpr126^st49/st49 mutant fish showing expression of krox20, mbp and sox10. Fish received either a control pulse of DMSO or 50 μM FSK from 50 to 55 hpf. Mutants that received the FSK treatment show continued expression of krox20 and mbp at 3 months of age, whereas control treated mutants show nearly undetectable levels of both genes. sox10 was expressed at the same level in all samples. (B) qPCR analysis of the mRNA expression levels of krox20, mbp and sox10 in dissected PLL nerves from 6-month-old zebrafish that received treatments as described in A. Fold differences are reported relative to the mutants. Note that the scale is logarithmic. The fold difference between FSK-treated mutants and control mutants for krox20 and mbp was 3.7 and 49.7, respectively. Significance with two-tailed Student’s t-test: ^*P<0.05; n.s., not significant. Error bars represent s.e.m.