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Fig. 6
Diurnal time course of per1b, bmal1 and luciferase mRNA expression. Schematic (upper left corner) indicates the level of the sections shown in the figure. Rows: time points at which fish were sacrificed (ZT3, ZT9, ZT15, ZT21, and ZT32 on the following day). Rows show in situ hybridizations with antisense probes for the indicated genes. Rows 4 and 5 show magnifications of the luciferase staining in the optic tectum (TeO, PGZ) and ventral hypothalamus regions (Hv), respectively, as indicated by the boxes in row 3. Scale bar, 250 μm and 125 μm for high magnifications.
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Reprinted from Developmental Biology, 380(2), Weger, M., Weger, B.D., Diotel, N., Rastegar, S., Hirota, T., Kay, S.A., Strähle, U., and Dickmeis, T., Real-time in vivo monitoring of circadian E-box enhancer activity: A robust and sensitive zebrafish reporter line for developmental, chemical and neural biology of the circadian clock, 259-73, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.