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Fig. 8
Differential expression of zebrafish prominin-1a– and b–positive cells in the posterior dorsal telencephalon.
(A–C) Cryosections of 3-month-old adult brain from BrdU-treated zebrafish were processed for ISH using an antisense DIG-labeled probe either against prominin-1a (A; prom1a), prominin-1b (B; prom1b) or musashi-1 (C; msi1). Proliferating cells were observed by immuno-detection of BrdU (brown). Position of the two coronal cross section profiles (I, II) encompassing the posterior dorsal telencephalic area is indicated on the cartoon adapted from a neuroanatomical atlas by Wulliman and colleagues [104], and the distribution of proliferative events (brown dots) is schematically shown. (A) Prominin-1a–positive cells are distributed along the posterior medial, dorsal and lateral aspects of the dorsal telencephalic surface proliferation zone (yellow arrows, see inset), and in the extraventricular brain parenchyma of the dorsal telencephalon (dT, asterisks) as well in the periventricular preoptic nucleus (PPa, blue arrows). (B) No prominin-1b–positive cells are detected within the dorsal telencephalic and preoptic proliferative zones (brown arrows). (C) Prosencephalic msi1–positive cells are found in a narrow ventricular stripe encompassing the proliferating subdivisions of the telencephalon, and that of the preoptic area and recessus opticus (Ro) of the diencephalic ventricle (green arrows). Proliferative zones 3 and 4 correspond to dorsal telencephalon and preoptic area, respectively. CO, chiasma opticum. Scale bars, A–C, 250 μm.