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Fig. S7
NICD-induced blastema expansion is reversible. (A-C′) Heat-shock cycles were applied over 5 days of regeneration (A-B′) or 12 days (C,C′). Black line indicates time of regeneration. Pink line indicates time of heat-shock treatment (1 hour of heatshock every 3 hours for 5 or 12 days). Arrows indicate regenerating radials. Arrowheads indicate expanded blastemas. Regeneration is inhibited in Tg(UAS:NICD) (B,C) fins but not in wild-type fins (A). Regeneration reverts to normal when heat-shock treatment is stopped (A′,B′, arrows) but the blastema remains close to the amputation plane (arrowhead) when heat-shocked continued up to 12 days (C′). Scale bars: 100 μm. Dashed lines mark the amputation plane.