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Fig. 8 Levels of def transcripts and Def protein in the adult liver of Tg-I, Tg-II, Tg-III and Tg-IV.
(A–D) The def transcript levels in Tg-I, Tg-II and Tg-III (A) or in Tg-I and Tg-IV (B) were determined by qPCR and Def protein levels by western blotting using an anti-Def monoclonal antibody (C and D). Interestingly, while the transcriptional expression of def showed a great range of difference in different genetic background (145, 27, 18 and 26 folds higher in Tg-I, Tg-II, Tg-III and Tg-IV, respectively, than in the WT) (A and B) the protein levels in these three lines did not differ in a corresponding scale (6.4, 2.8, 2.3 and 4.9 folds higher in Tg-I, Tg-II, Tg-III and Tg-IV, respectively, than in the WT) (C and D).