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Fig. 2 Tg-I expresses high level of def specifically in the liver.
(A) WISH analysis of def expression in Tg-I and wildtype (WT) embryos at 4.5 dpf using the full length def cDNA as a probe. lv: liver, in: intestine. (B) Western blot of Def in Tg-I and wildtype (WT) embryos at 4.5 dpf. GAPDH: loading control. (C) Immunostaining of Def (green) and the nucleolar marker Fibrillarin (Fib) (red) in the liver of Tg-I fish at 4.5. DAPI was used to stain the nucleoli.
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