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Fig. 4 Structure-function analyses of effect of vox activity on Cas function. (A-E,G-J) Zebrafish embryos were either left uninjected (A,G) or injected with the RNA indicated bottom right (B-E,H-J) and allowed to develop until 80% epiboly, then processed for in situ hybridization with sox17 probe. (F,K) qRT-PCR analysis as in Fig. 3. For statistical analyses, samples cas+vox or casΔN-vox or cas+ΔC-vox are compared with cas RNA-injected samples (F) and vox, EnR-ΔN-vox, VP16-ΔN-vox with uninjected samples (K); ***P<0.005, **P<0.05; error bars indicate s.d.
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