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Fig. 9 Treatment with forskolin rescues peripheral myelin gene expression in dync1h1 mutant larvae. Wild-type larvae treated with forskolin express egr2b (A) and mbp (B, C) RNA normally at motor nerves (mn, asterisks) and the pLLn (arrowhead). dync1h1 mutant larvae treated with forskolin express egr2b (D) and mbp (E, F) RNA indistinguishably from wild type. In forskolin-treated dync1h1/ larvae, Schwann cells associated with motor nerve (G) and pLLn axons (H) marked by anti-acetylated tubulin staining (red), express Mbp (green). Images in A-F are whole mount views of 3 dpf larvae, anterior to the left and dorsal up. Images in G and H are single z-planes captured from transverse sections at 6 dpf. Dashed line indicates the lateral edge of the fish. Scale bars equal 20 μm (A-F), 40 μm (G) and 5 μm (H).