Fig. 2

Fig. 2 Loss of Fgf signaling results in fewer parapineal cells. (A,B) Dorsal views of WT and fgf8a^x15 mutant zebrafish embryos labeled with gfi-1.2 (parapineal cells; black arrowheads). (C,D) Dorsal views showing expression of gfi-1.2 (black arrowheads) in WT larvae at 52 hpf that were treated between 24 and 30 hpf with either DMSO or SU5402. In A-D, dashed line encircles the pineal complex anlage. (E) fgf8a^x15 mutants displayed a significant reduction in parapineal cell number compared with WT (^***P=<0.0005 by t-test). (F) Reductions in parapineal cell numbers were observed in embryos treated with SU5402, particularly for 18-24 hpf and 24-30 hpf treatments (^***P<0.0005 by t-test). Error bars represent s.e.m. Scale bar: 25 μm.