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Fig. 3 etv5b loss-of-function does not affect the number of cells in other basal forebrain neuron populations. (A-E) Confocal maximum intensity projections showing dopaminergic (DA) neuron clusters (4/5/6 and 7) of the basal forebrain revealed by TH1 immunohistochemistry in controls and etv5b morphants. Ventral views, anterior left. (F,G) The number of TH1-immunoreactive cells in the different DA clusters at 72 hpf in controls and etv5b ATG (0.075 mM, F) or splice (0.15 mM, G) morphants expressed as percentage of control. (H-V) Expression of oxtl, avpl and sst3 in controls and etv5b morphants. Dorsal views, anterior left. Brackets in H, M and R indicate the population included in the analysis. (W,X) The number of cells expressing oxtl, avpl or sst3 at 72 hpf in controls and etv5b ATG (0.075 mM, W) or splice (0.15 mM, X) morphants expressed as percentage of control. n, total number of embryos analysed for each experiment. n.s., not significant. Scale bars: 50 μm.