IMAGE

Fig. 3

ID: ZDB-IMAGE-130117-18
Publication: Liu et al., 2012 - Prdm14 acts upstream of islet2 transcription to regulate axon growth of primary motoneurons in zebrafish
All Figures: Figures for Liu et al., 2012

Image

Figure Caption

Fig. 3 prdm14 expression is reduced in slg mutant and splmo morphant embryos. (A) The zebrafish prdm14 genomic locus. The transposon is inserted in the first intron. Primers used in C and D are indicated. (B,C) RNA in situ hybridization (B, arrowheads) and RT-PCR (C) indicate that prdm14 expression is greatly reduced in slg mutant embryos. ODC, ornithine decarboxylase 1 internal standard. (D) prdm14 splicing is disrupted in splmo morphants. (E) Real-time RT-PCR results indicate that prdm14 expression is reduced to ~75% in slg heterozygotes and to ~20% in homozygotes. Error bars indicate s.e.m. of triplicate experiments. (F) Immunoprecipitation (IP) showing that Prdm14 protein levels are greatly decreased in slg homozygotes and splmo morphants. Scale bar: 500 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development