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Fig. 3 FGF signaling is down regulated in muscle pioneer precursor domain.
(A-B) Asymmetric expression of erm at 9-somites as determined by ISH on (B-B′′) serial sagittal sections and (A) cross-section. Panel (B′*) represents a high magnification of the region in box in (B′) panel. The different positions of sagittal sections are represented in (A). Expression of erm is absent in anterior adaxial cells (arrow heads). (C) spry4 expression in a 9-somite embryo determined by ISH on a cross section of the anterior somitic region. (D-D′′′) Expression of spry4 mRNA (D′, red), sMyHC (D′′, green) and DAPI (D-D′′, blue) in 9-somite embryos, dorsal view. spry4 expression in the anterior adaxial cells is indicated (arrow heads). D′-D′′′ high magnification view of the area boxed in D.