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Fig. S3 dmrt2a mRNA is a target of Celf1. (A,B) celf1 (left) or dmrt2a (right) expression in zebrafish embryos at 12-14 hpf. (A) Dorsal view of whole-mount embryos. Scale bar: 200 μm. (B) Dorsal view of flatmounted embryos. Arrows mark the position of Kupffer’s vesicle. (C) Sequence of the 3UTR of dmrt2a mRNA. Red letters are the putative Celf1-binding site including UGU repeats (underlined), and U- and A-rich sequences. The 35 nucleotides were used as a probe named dmrt2a_wildtype for an in vitro binding assay (see also Fig. 4A). (D,E) The putative Celf1-binding sites including UGU repeats (underlined) and U- and A-rich sequences were found within 3UTR of myod (D) or rbpj (E) mRNA, but sequence homology was low among species.