Celf1 regulation of dmrt2a is required for somite symmetry and left-right patterning during zebrafish development
- Authors
- Matsui, T., Sasaki, A., Akazawa, N., Otani, H., and Bessho, Y.
- ID
- ZDB-PUB-120822-15
- Date
- 2012
- Source
- Development (Cambridge, England) 139(19): 3553-3560 (Journal)
- Registered Authors
- Matsui, Takaaki
- Keywords
- none
- MeSH Terms
-
- Base Sequence
- Chlorocebus aethiops
- Mice
- Molecular Sequence Data
- Cells, Cultured
- Transcription Factors/genetics*
- Transcription Factors/metabolism
- Transcription Factors/physiology
- Embryo, Nonmammalian
- RNA-Binding Proteins/antagonists & inhibitors
- RNA-Binding Proteins/genetics
- RNA-Binding Proteins/physiology*
- Gene Knockdown Techniques
- DNA-Binding Proteins/genetics*
- DNA-Binding Proteins/metabolism
- DNA-Binding Proteins/physiology
- CELF1 Protein
- Animals, Genetically Modified
- Somites/embryology*
- Somites/metabolism
- Body Patterning/genetics*
- Gene Expression Regulation, Developmental/physiology
- Animals
- COS Cells
- Zebrafish Proteins/antagonists & inhibitors
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish Proteins/physiology*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 22899848 Full text @ Development
RNA-binding proteins (RBPs) bind to numerous and diverse mRNAs to control gene expression post-transcriptionally, although the in vivo functions of specific RBP-mRNA interactions remain largely unknown. Here, we show that an RBP named Cugbp, Elav-like family member 1 (Celf1) controls expression of a gene named doublesex and mab-3 related transcription factor 2a (dmrt2a), which is essential for somite symmetry and left-right patterning during zebrafish development. Celf1 promotes dmrt2a mRNA decay by binding to UGU repeats in the 32UTR of dmrt2a mRNA such that celf1 overexpression reduces the amount of dmrt2a mRNA, leading to asymmetric somitogenesis and laterality defects. Furthermore, blocking the Celf1-dmrt2a mRNA interaction by a target protector morpholino alleviates failures in somite symmetry and left-right patterning that are caused by celf1 overexpression. Our results therefore demonstrate that Celf1-dependent fine-tuning of dmrt2a expression is essential for generating bilateral symmetry of somites and left-right asymmetric patterning during zebrafish development.