Fig. 7

Fig. 7 Neural crest progenitor and cranial ganglia phenotypes of sf3b1^b460 mutants. There is reduced melanoblast expression of mitfa (A,B) and nearly absent melanoblast expression of dct (C,D) in sf3b1^b460 mutants. (E-J) Pharyngeal arches are dramatically reduced in sf3b1^b460 mutant embryos. (E-H) Lateral view of whole-mount in situ embryos with dlx2 antisense RNA probe, arch progenitors denoted by arrows. (I,J) Dorsal view of flat-mounted in situ embryos with dhand antisense RNA probe, pharyngeal arches 1 and 2 are decreased and 3, 4 and 5 are absent in sf3b1^b460 mutants. (K-N) the cranial ganglia are also affected in sf3b1^b460 mutants. (K,L) Whole-mount antibody staining with zn-12. Arrows in (K,L) show the trigeminal ganglia. (M,N) Whole-mount antibody staining with anti-acetylated tubulin antibody. Arrows in (M,N) show the decreased and disorganized trigeminal ganglia in sf3b1^b460 mutants compared to wildtype siblings. (O,P) Crestin expressing cells are present in sf3b1^b460 mutant embryos. However, these cells are reduced in numbers, particularly in the cranial region and fail to migrate normally in the sf3b1^b460 mutant (P) compared to wildtype embryos (O).