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Fig. S7
The effect of β-arrestin knockdown on Cxcr7b level. Western blot analysis showing the level of Cxcr7b-EGFP and α-tubulin (loading control) in whole embryo lysates at 11 hpf. Embryos were injected at 1-cell stage with Cxcr7b-EGFP mRNA and control (COMO) or β-arrestin MO (βArrMOs). Cxcr7b-EGFP protein level was detected using anti-GFP antibody (Clontech, 632377). Endogenous α-tubulin was detected using anti-tubulin antibody (Acris, C0379). Graph represents the ratios between Cxcr7b and α-tubulin band intensities as determined by 2D densitometry (AIDA, BioImaging).
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