Fig. 1

Fig. 1 mRNA expression patterns of scube1 and scube3. (A–I) scube1 mRNA expression. (A) Maternal expression of scube1 in 256 cell embryo, (B) scube1 is expressed in a stripe of cells corresponding to the developing notochord (nc) at <90% epiboly. (C) At 15 somites, scube1 is expressed in the somitic mesoderm (sm), and notochord. (D, E) At 26 somites scube1 expression is in the forebrain (fb), olfactory bulb (ob), midbrain (mb), hindbrain (hb), rhombomeres (bracket), with diffuse staining present in notochord and floorplate (fp) of neural tube [E closeup of axial somites in embryo equivalent to D]. (F–I) Sections of 24 hpf embryos. (F) scube1 expression in forebrain, cerebellar neurons (cb), lens (ln), optic cup (oc) with strong expression in the olfactory (ol) forebrain region. (G, I) scube1 is expressed throughout the neural tube (nt) with strong floorplate and lateral nt expression. (H, I) scube1 somitic mesoderm expression in tail sections. (J–P) scube3 mRNA expression. (J) Maternal expression of scube3 in 256 cell embryo. (K) scube3 expression in the floorplate, notochord, somitic mesoderm, and PSM at 15 somites. (L, M) Extensive neural expression (telencephalon te, diencephalon, di, midbraim, mb, and hindbrain, hb), expression in the floorplate, somites and diffusely in the notochord at 26 somites. (M) Magnification of tail region in (L) showing strong ventral floorplate staining and weak notochord staining. N–P sections of 24 hpf embryos. (N) scube3 is expressed in the cerebellum, the lens of the eye and the optic cup and weakly in the forebrain. (O) scube3 expression is strong in sclerotome ventral to the notochord. (P) scube3 expression throughout neural tube, strong expression in floorplate and weak notochord expression. Scale Bars: 100 μm. (C, K) Dorsal view, (D, E, L, M) lateral view (F, H, N) longitudinal sections, (G, I, O, P) transverse sections.