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Fig. 4 Notum 1a is required for proper neural tube patterning. (A,B) Compared with uninjected controls at 24 hpf (A), notum1a expression is expanded in the neural tubes of Notum 1a-depleted embryos (P=7.8 × 10–9) (B). (C,D) Similarly, compared with uninjected embryos at 24 hpf (C), msxc is expanded in notum1a MO-injected embryos (P=2.0 × 10–4) (D). (E,F) msxc expression is lost in the dorsal neural tube of heat-shocked Tg(hs:notum1a) embryos at 24 hpf (F). Note the normal expression of ntl in the notochord. (G-J) Loss of notum1a leads to ectopic dkk1 expression in the neural tube. Although dkk1 is not observed in the neural tube of uninjected embryos at 24 hpf (G), it was faintly (I) or intensely (H) expressed within the neural tube in the majority of notum1a MO-injected embryos (J).