Fig. 1 Expression of miR-2188 and Nrp2a in zebrafish embryos.
A) ISH of miR-2188 at 24 hpf showed low expression on the trunck and tail, however it increased at 48 hpf between the somite boundaries. Arrows indicate hybridization signal. B) Nrp2a ISH at 24 and 48 pf. At 24hpf Nrp2a was predominantly expressed in the head and somitic regions, while at 48 hpf its expression was restricted to the brain and romboencephalon. There was an indirect correlation between miR-2188 and Nrp2a expression. Increasing miR-2188 levels through development were accompanied by decreasing Nrp2a expression between the somite boundaries. All embryos are in lateral view, anterior to left. Microscopic pictures were taken with 6,3x magnification.