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Fig. S4 Injection of morpholinos against ring1b phenocopies the ring1b developmental phenotype. Lateral views of embryos at 72 hpf. MO1- and MO2-injected embryos display the heart edema (lines) and jaw abnormalities (arrows) similar to ring1b mutants (A-C). Expression of tbx5 (E,F), hand2 (H,I) and fgf24 (K,L) in ring1b MO-injected embryos at 48 hpf, shows the same pattern as the one in ring1b mutants. No Ring1b protein is detected in lysates from ring1b MO-injected embryos (M). Histone H3 serves as loading control.
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