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Fig. S5
Additional information about zebrafish PABP. (A) In situ hybridization detecting pabpc1a mRNA (top), pabpc1b mRNA (middle) and pabpc4 (bottom) in 24-hpf zebrafish embryos (purple). (B) Validation of the PABP antibody used in this study. Western blotting was performed with extracts from 24-hpf embryos injected with myc-tagged pabpc1a mRNA, myc-tagged pabpc1b or myc-tagged pabpc4 mRNA. The PABP antibody detected myc-PABPC1a, myc-PABPC1b and myc-PABPC4 (Upper). The expression of myc-tagged proteins was also detected using the anti-myc antibody (Middle), and eIF5 served as a loading control (Lower).
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