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Fig. 2 Rescue of neural apoptosis and MHB marker loss in grhl2b morphants by mouse Grhl2 mRNA. (A-E) Cell death rescue (arrows) demonstrated by bright-field microscopy (A,B, insets) and TUNEL staining (C-E) around the MHB region (M) of embryos sequentially injected with MO:grhl2b and either control lacZ mRNA (A,C) or mouse Grhl2 mRNA (B,D). TUNEL staining reveals few apoptotic cells in an embryo injected with MO:control (E). (F-I) Rescue (arrows) of MHB marker expression (eng2a, pax2a) in embryos sequentially injected with MO:grhl2b and either lacZ mRNA (F,G) or mouse Grhl2 mRNA (H,I). (J-O) Persistent defects in MHB folding (J,K) and marker expression (eng2a, pax2a) (L-O, arrows) in p53–/– fish injected with MO:grhl2b compared with MO:control. TUNEL assay confirms substantially reduced apoptosis in p53–/– fish injected with MO:grhl2b (J, inset; compare with C)