Fig. 2
Expression of zebrafish Lgals1-L2 and Lgals1-L3. Whole-mount in situ hybridization on 48-h zebrafish embryos. (A) Lgals1-L2 is strongly expressed in the eyes around the lens (arrow) and in the ventricular zone in the head (arrowheads). (B) Lgals1-L3 expression is less restricted but does overlap with L2 expression around the lens (arrow) and in the ventricular zone (arrowheads). (C–E) Cross-sections at the level of the midbrain of whole-mount in situ hybridizations of Lgals1-L2 (C) (Inset is photographed from more anterior section), Lgals1-L3 (D), and VE-cadherin (E). Expression of both Lgals1-L2 and L3 is observed in blood vessels in the brain (arrowhead in C and D) and the retinal vessels (arrow in C and D) and colocalizes with the expression of EC marker VE-cadherin.