ZFIN Image: Jurynec et al., 2008, Fig. S1

Image

Figure Caption

Fig. S1

SepN genomic structure and morpholino inhibition of SepN expression. (a) Genomic structure of the zebrafish sepN locus containing conserved elements including a selenocysteine codon (Sec), a Sec Redefinition Element (SRE), and a selenocysteine insertion sequence (SECIS) element. * indicates stop codon; red bars indicate sbMOs; arrows indicate primers used to detect sepN transcripts by RT-PCR. (b) RT-PCR analysis of sepN transcripts in 24 hpf WT, MO1- or MO2-injected embryos. *,WT sepN amplicon; arrows, intron-included amplicons; m, markers. (c and d) sepN RNA levels are reduced in MO1-injected embryos. Lateral views with rostral left.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA