Fig. 4
Noncanonical Wnt signaling and NC migration. (A–C) Injection of both dN dvl mRNA and bbs8 MO individually results in inhibition of NCC migration relative to controls. (D) Coinjection of 3 ng of bbs8 MO with dN dvl results in partial rescue of NCC migration by H4-fold relative to bbs8 morphants. (E) Coinjection of dN dvl mRNA with 2 ng of bbs8 MO fails to rescue NCC migration. (F) Injection of 50 pg of myr/pal dvl causes inhibition of NCC migration. (G) Coinjection of myr/pal dvl with 3 ng of bbs8 MO also partially rescues the inhibited NC migration. (H) Graph quantifying the number of streams of crestin-positive NCCs migrating through the trunk. (Scale bar: 200 μm.)