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Fig. 3
Immunohistochemical detection of BrdU incorporation and germline markers in ovarian tissue, indicating germline cell proliferation, following 14 days Mtz treatment (5 mM). A: Clusters of BrdU-positive cells were readily detectable at end of treatment period. B: Immunohistochemical identification of a candidate oogonial stem cell (arrowhead) positive for both BrdU (green) and the germline marker Vasa (Red); arrows indicate BrdU-positive somatic cells.
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