ZFIN ID: ZDB-PUB-110719-33
A transgenic zebrafish model of targeted oocyte ablation and de novo oogenesis
White, Y.A., Woods, D.C., and Wood, A.W.
Date: 2011
Source: Developmental dynamics : an official publication of the American Association of Anatomists   240(8): 1929-1937 (Journal)
Registered Authors:
Keywords: zebrafish, oogenesis, transgenics, ovary, oocyte, stem cell, ablation
MeSH Terms:
  • Animals
  • Animals, Genetically Modified*
  • Anti-Infective Agents/pharmacology
  • Female
  • Male
  • Metronidazole/pharmacology
  • Models, Animal*
  • Oocytes/cytology
  • Oocytes/drug effects
  • Oocytes/physiology*
  • Oogenesis/physiology*
  • Ovary/anatomy & histology
  • Ovary/drug effects
  • Ovary/pathology
  • Prodrugs/pharmacology
  • Signal Transduction/physiology
  • Zebrafish/anatomy & histology
  • Zebrafish/physiology*
PubMed: 21761478 Full text @ Dev. Dyn.
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ABSTRACT
We describe here a novel transgenic zebrafish, Tg(zpc:G4VP16/UAS:nfsB-mCherry) that effectively demonstrates the targeted oocyte ablation in the adult zebrafish ovary. This transgenic line expresses bacterial nitroreductase enzyme (nfsB) under the control of the oocyte-specific zona pellucida C (zpc) gene promoter. Adult transgenic females exposed to the prodrug metronidazole demonstrated near-complete ablation of growing oocytes, resulting in ovarian degeneration and complete cessation of reproductive function. Within 4 weeks of prodrug removal, treated fish demonstrated complete anatomical regeneration of the ovary and, within 7 weeks, ovarian function (fertility) was fully restored. Together, these results demonstrate functional renewal of the oocyte pool in the adult zebrafish ovary. Accordingly, this transgenic zebrafish model system provides a novel means to investigate ovarian growth dynamics in a genetically tractable vertebrate, and may be useful for evaluating signaling interactions that regulate gonadal development processes such as de novo oogenesis.
ADDITIONAL INFORMATION