|ZFIN ID: ZDB-PUB-110719-33|
A transgenic zebrafish model of targeted oocyte ablation and de novo oogenesis
White, Y.A., Woods, D.C., and Wood, A.W.
|Source:||Developmental dynamics : an official publication of the American Association of Anatomists 240(8): 1929-1937 (Journal)|
|Keywords:||zebrafish, oogenesis, transgenics, ovary, oocyte, stem cell, ablation|
|PubMed:||21761478 Full text @ Dev. Dyn.|
White, Y.A., Woods, D.C., and Wood, A.W. (2011) A transgenic zebrafish model of targeted oocyte ablation and de novo oogenesis. Developmental dynamics : an official publication of the American Association of Anatomists. 240(8):1929-1937.
ABSTRACTWe describe here a novel transgenic zebrafish, Tg(zpc:G4VP16/UAS:nfsB-mCherry) that effectively demonstrates the targeted oocyte ablation in the adult zebrafish ovary. This transgenic line expresses bacterial nitroreductase enzyme (nfsB) under the control of the oocyte-specific zona pellucida C (zpc) gene promoter. Adult transgenic females exposed to the prodrug metronidazole demonstrated near-complete ablation of growing oocytes, resulting in ovarian degeneration and complete cessation of reproductive function. Within 4 weeks of prodrug removal, treated fish demonstrated complete anatomical regeneration of the ovary and, within 7 weeks, ovarian function (fertility) was fully restored. Together, these results demonstrate functional renewal of the oocyte pool in the adult zebrafish ovary. Accordingly, this transgenic zebrafish model system provides a novel means to investigate ovarian growth dynamics in a genetically tractable vertebrate, and may be useful for evaluating signaling interactions that regulate gonadal development processes such as de novo oogenesis.